Loss of cationic peptides with agarose gel-immobilized tris[2- carboxyethyl]phosphine (TCEP)

Reaction of Tris(2-carboxyethyl)phosphine (TCEP) with maleimide and alpha-haloacyl groups: anomalous elution of TCEP by gel filtration.

Agarose gel electrophoresis.

MATERIALS 6x Gel-loading buffer Agarose solutions (please see Step 3) DNA samples DNA size standards Samples of DNAs of known size are typically generated by restriction enzyme digestion of a plasmid or bacteriophage DNA of known sequence. Alternatively, they are produced by ligating a monomer DNA fragment of known size into a ladder of polymeric forms. DNA staining solution For a discussion of...

Agarose gel electrophoresis.

Agarose gel electrophoresis is used to separate DNA or RNA molecules based upon their size.

Alkaline agarose gel electrophoresis.

INTRODUCTION Alkaline agarose gels are run at a pH that is sufficiently high to denature double-stranded DNA. The denatured DNA is maintained in a single-stranded state and migrates through the alkaline gel as a function of its size. Alkaline agarose gels are used chiefly to measure the size of first and second strands of cDNA (Construction of cDNA Libraries Stage 1: Synthesis of First-strand c...

Kinetic Model for Whey Protein Hydrolysis by Alcalase Multipoint- Immobilized on Agarose Gel Particles

Partial hydrolysis of whey proteins by enzymes immobilized on an inert support can either change or evidence functional properties of the produced peptides, thereby increasing their applications. The hydrolysis of sweet cheese whey proteins by alcalase, which is multipoint-immobilized on agarose gel, is studied here. A MichaelisMenten model that takes into account competitive inhibition by the ...